Description
|
Assay range |
3.125-200 ng/ml |
|
Sensitivity |
3.0 ng/ml |
|
Specificity |
No cross-reaction with other related substances detected |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
50 µL final volume, dilution factor varies on samples |
|
Sample Type |
Urine and cell culture supernatants |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Bovine Plasminogen standard: 1 vial
2. One 96-well plate coated with Bovine Plasminogen Ab
3. Diluent buffer (10x): 30 mL – 1
4. Biotinylated Bovine Plasminogen Ab (100x): 80µL
5. Streptavidin-HRP(100x): 80 µL
6. TMB developing agent: 8 mL x1
7 . Stop solution: 12 mL x1
8. Washing solution (20x): 30 mL x2
Background
Plasminogen is a single chain glycoprotein zymogen that is primarily synthesized in the liver and circulated in plasma with a molecular weight of 90 kDa. Human Plasminogen contains an N-terminal activation peptide, five characteristically folded kringle domains, and a peptidase S1 domain. Cleavage of the activation peptide produces mature Plasminogen, which can be further cleaved between Arg580 and Val581 by tissue plasminogen activator (tPA) to produce the disulfide-linked two-subunit enzyme plasmin that plays a key role in breakdown of fibrin clots. The plasminogen system plays a role in macrophage recruitment, arterial stenosis, atherosclerosis, aneurysm formation, skin and corneal wound healing, glomerulonephritis, and neovascularization.
