Description
Mouse Interleukin 1β,IL-1β ELISA Kit
For protocols and availability questions, please fill out our contact us form and make sure to include your company name, business email, and phone number. We will contact you within 24 hours.
Different lot may vary, please follow the instruction coming with the kit.
Data Sheet
| Assay Range | 31.2 – 2000 pg/ml |
| Assay Time | 4.5 hours |
| Assay Type | Sandwich ELISA |
| Background | Interleukin 1 beta (IL-1β) also known as leukocytic pyrogen, leukocytic endogenous mediator, mononuclear cell factor, lymphocyte activating factor and other names, is a cytokine protein that in humans is encoded by the IL1B gene. There are two genes for interleukin-1 (IL-1): IL-1 alpha and IL-1 beta (this gene). IL-1β precursor is cleaved by cytosolic caspase 1 (interleukin 1 beta convertase) to form mature IL-1β.
The fever-producing property of human leukocytic pyrogen (interleukin 1) was purified by Dinarello in 1977 (PNAS) with a specific activity of 10–20 nanograms/kg. In 1979, Dinarello reported that purified human leukocytic pyrogen was the same molecule that was described by Igal Gery in 1972. He named it lymphocyte-activating factor (LAF) because it was a lymphocyte mitogen. It was not until 1984 that interleukin 1 was discovered to consist of two distinct proteins, now called interleukin-1 alpha and interleukin-1 beta. IL-1β is a member of the interleukin 1 family of cytokines. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
| Kit Type | Colorimetric |
| Sample Type | serum, Plasma, Cell culture supernatant |
