Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MGSMNSGRPE TMENLPALYT IFQGEVAMVT DYGAFIKIPG CRKQGLVHRT HMSSCRVDKP SEIVDVGDKV WVKLIGREMK NDRIKVSLSM KVVNQGTGKD LDPNNVIIEQ EERRRRSFQD YTGQKITLEA VLNTTCKKCG CKGHFAKDCF MQPGGTKYSL IPDEEEEKEE AKSAEFEKPD PTRNPSRKRK KEKKKKKHRD RKSSDSDSSD SESDTGKRAR HTSKDSKAAK KKKKKKKHKK KHKE |
| Description | ZCCHC17 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 264 amino acids (1-241a.a.) and having a molecular mass of 30.0 kDa (Molecular weight on SDS-PAGE will appear higher). ZCCHC17 is fused to a 23 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The ZCCHC17 protein solution (0.25mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0), 100mM NaCl, 2mM DTT, 0.1mM PMSF and 20% glycerol. |
| Protein Background | ZCCHC17 protein interacts with PNN and associates with the 60S ribosomal subunit. Universally expressed, ZCCHC17 is localizing to the nucleolus, and takes part in ribosome maturation and biogenesis. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | Zinc finger CCHC domain containing 17, PS1D, pNO40, HSPC251, Pnn-interacting nucleolar protein, Putative S1 RNA-binding domain protein, RP11-266K22.1, Nucleolar protein of 40 kDa, PS1D protein. |
