Description
Assay Range
0.5 – 16 U/L
Sensitivity
0.1 U/L
Assay Principle
Sandwich ELISA
Storage
Store at 2 – 8â°C
Sample Type
Serum, plasma, body fluids, tissus lysates
Size
96T
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant Rat iNOS standard: 0.5ml/vial x 6.
2. One 96-well plate precoated with anti-Rat iNOS antibody.
3. Sample diluent buffer: 6 ml
4. HRP conjugated anti-Rat iNOS antibody: 10 ml.
5. Plate sealer: 2
6. 20 x Wash buffer: 25 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6 ml.
