Description
Data Sheet
| Amino acid sequence | MPPKFKRHLN DDDVTGSVKS ERRNLLEDDS DEEEDFFLRG PSGPRFGPRN DKIKHVQNQV DEVIDVMQEN ITKVIERGER LDELQDKSES LSDNATAFSN RSKQLRRQMW WRGCKLEHHH HHH. |
| Antibody Properties | Reconstitute with sterile H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Description | VAMP4 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 123 amino acids (1-115 a.a.) and having a molecular mass of 14.5kDa.VAMP4 is fused to 8 amino acids His Tag at C-terminus and purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The VAMP4 protein solution contains 20mM Tris-HCl buffer (pH 8.0), 0.1M NaCl, and 20% glycerol. |
| Protein Background | VAMP4 belongs to the synaptobrevin family who are involved in docking and/or fusion of vesicles with cell membrane. VAMP4 is enriched in the trans-Golgi network and may be a factor in the trans-Golgi network-to-endosome transport. VAMP4 is involved in the pathway which functions to eliminate an inhibitor (most likely synaptotagmin-4) of calcium-triggered exocytosis during the maturation of secretory granules. Synaptobrevins, syntaxins, and SNAP25 are the chief components of a protein complex involved in the docking and/or fusion of synaptic vesicles with the presynaptic membrane. |
| Protein content | For intra-cellular staining use 10ul per 1,000,000 cells. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Reconstitution | 1 mg/ml in PBS (after reconstitution). |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | Vesicle-associated membrane protein 4, VAMP-4, VAMP4, VAMP24. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
