Description
Data Sheet
| Applications | Immunoassays and western blot. |
| Description | Recombinant Single Chain Cardiac Troponin I-C 2nd generation produced in E.Coli is a single, non-glycosylated, polypeptide chain (aa28-110-Linker-cTnC), having a molecular weight of 30kDa.1mg of scIC2 is equivalent to 0.82mg of full length human cardiac TNI on a molar basis.The scIC2 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The protein solution contains 20mM Tris-HCl, 250mM NaCl and 50% glycerol, pH 7.5. |
| Protein Background | Troponin Complex is a heteromeric protein playing an important role in the regulation of skeletal and cardiac muscle contraction. It consists of three subunits, Troponin I, Troponin T and TroponinC. Each subunit is responsible for part of Troponin Complex function. E.g. Troponin I inhibits ATP-ase activity of acto-myosin.Troponin T and Troponin Iare presented in cardiac muscles in different forms than in skeletal muscles. Purified subunits of rcTnI, rcTnC and rcTnT are recomplexed in vitro under appropriate conditions. |
| Purity | Greater than 91.0% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered colourless liquid formualtion. |
| Stability | scIC2 although stable at 10°C for 7 days, should be stored below -18°C. Please prevent freeze-thaw cycles. |
| Synonyms | Troponin I cardiac muscle, Cardiac troponin I, TNNI3, TNNC1, CMH7, RCM1, cTnI, CMD2A, MGC116817, Troponin C slow skeletal and cardiac muscles, TN-C, TNNC1, TNNC, TNC, CMD1Z. |
