Description
Data Sheet
| Amino acid sequence | ALDTNYCFRN LEENCCVRPL YIDFRQDLGW KWVHEPKGYY ANFCSGPCPY LRSADTTHST VLGLYNTLNP EASASPCCVP QDLEPLTILY YVGRTPKVEQ LSNMVVKSCK CS. |
| Biological Activity | The activity is determined by the ability to induce chondrogenic differentiation. |
| Description | TGF-beta 3 Human Recombinant produced in E.Coli is a disulfide-linked homodimeric, non-glycosylated, polypeptide chain containing two 112 amino acid chains and having a total molecular mass of 25.5kDa. The TGF-b 3 is purified by standard chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The protein solution contains 20% Ethanol and 0.12% Acetic acid. |
| Protein Background | Transforming growth factor betas (TGF Betas) mediate many cell-cell interactions that occur during embryonic development. Three TGF Betas have been identified in mammals. TGF Beta 1, TGF Beta 2 and TGF Beta 3 are each synthesized as precursor proteins that are very similar in that each is cleaved to yield a 112 amino acid polypeptide that remains associated with the latent portion of the molecule. |
| Purity | Greater than 96.0% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear solution. |
| References | 1.Title: Altering the Architecture of Tissue Engineered Hypertrophic Cartilaginous Grafts Facilitates Vascularisation and Accelerates MineralisationPublication: DOI: 10.1371/journal.pone.0090716, March 04, 2014Link: http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.00907162. Title: Oxygen tension differentially regulates the functional properties of cartilaginous tissues engineered from infrapatellar fat pad derived MSCs and articular chondrocytesPublication: Osteoarthritis and Cartilage 18.10 (2010): 1345-1354.Link: http://www.sciencedirect.com/science/article/pii/S1063458410002293 |
| Stability | TGF-beta 3 although stable at room temperature for 1 week, should be stored at 4°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). |
| Synonyms | Transforming Growth Factor-beta3, TGFB3, ARVD, FLJ16571, TGF-beta3. |
