Description
Data Sheet
| Biological Activity | The biological activity measured by the ability of fibrin lysis in agarose plate was found to be 50,000IU/mg. |
| Description | Staphylokinase Recombinant produced in E.Coli is a non-glycosylated polypeptide chain containing 136 amino acids and having a molecular weight of 16kDa.The Staphylokinase is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | Lyophilized from a 0.2um filtered concentrated solution in PBS, pH 7.4. |
| Protein Background | Staphylokinase (SAK) is a 136-amino acidenzymefrom Staphylococcus aureus. It is positively regulated by the “agr” gene regulator. It activates plasminogen, which in turn can degrade various host proteins during infection. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| References | Title: Staphylokinase production by clinical Staphylococcus aureus strainsPublications: Pol J Microbiol 56 (2007): 97-102.Link: http://www.researchgate.net/publication/6187424_Staphylokinase_production_by_clinical_Staphylococcus_aureus_strains |
| Solubility | It is recommended to reconstitute the lyophilized SAKin sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized SAK although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution SAK should be stored at 4°C between 2-7 days and for future use below -18°C.Please prevent freeze-thaw cycles. |
| Synonyms | Staphylokinase, SakSTAR, Neutral proteinase, Protease III, SAK. |
