Description
Data Sheet
| Amino acid sequence | MADVLDLHEA GGEDFAMDED GDESIHKLKE KAKKRKGRGF GSEEGSRARM REDYDSVEQD GDEPGPQRSV EGWILFVTGV HEEATEEDIH DKFAEYGEIK NIHLNLDRRT GYLKGYTLVE YETYKEAQAA MEGLNGQDLM GQPISVDWCF VRGPPKGKRR GGRRRSRSPD RRRRLEHHHH HH. |
| Description | RBM8A produced in E.Coli is a single, non-glycosylated polypeptide chain containing 182 amino acids (1-174 a.a.) and having a molecular mass of 20.9kDa.RBM8A is fused to an 8 amino acid His-tag at C-terminus & purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The RBM8A solution (0.5mg/ml) contains 20mM Tris-HCl buffer (pH8.0), 10% glycerol, 0.1M NaCl and 1mM DTT. |
| Protein Background | RBM8A belongs to the EJC (The exon junction complex) which is involved in mRNA export, cytoplasmic localization and nonsense mediated mRNA decay. RBM8A is able to communicate to the cytoplasm the processing history of the mRNA, including the position of the removed introns. Even though RBM8A shuttles to the cytoplasm, it is mostly detected in the nucleus and is colocalized with oskar mRNA at the posterior pole of the cell. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | RNA-binding protein 8A, Binder of OVCA1-1, BOV-1, RNA-binding motif protein 8A, RNA-binding protein Y14, Ribonucleoprotein RBM8A, RBM8A, RBM8, Y14, ZNRP, RBM8B, ZRNP1, BOV-1A, BOV-1B, BOV-1C, MDS014. |
