Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MREYKLVVLG SGGVGKSALT VQFVQGIFVE KYDPTIEDSY RKQVEVDCQQ CMLEILDTAG TEQFTAMRDL YMKNGQGFAL VYSITAQSTF NDLQDLREQI LRVKDTEDVP MILVGNKCDL EDERVVGKEQ GQNLARQWCN CAFLESSAKS KINVNEIFYD LVRQINRKTP VEKKKPKKKS C. |
| Description | RAP1A Recombinant E.coli produced in E.Coli is a single, non-glycosylated polypeptide chain containing 201 amino acids (1-181 a.a.) and having a molecular mass of 22.8 kDa. The RAP1A is fused to 20 amino acid His-Tag at N-terminus and purified by conventional chromatography. |
| Expression host | Escherichia Coli. |
| Formulation | 0.25mg/ml solution containing 20mM Tris HCL pH-8.0, 5mM DTT, 0.2M NaCl & 40% glycerol. |
| Protein Background | RAP1A is part of the family of RAS-related proteins which possess 50% amino acid identity with the classical RAS proteins and have various structural characteristicsin common. RAP proteins differ from RAS proteins in their 61st amino acid, glutamine in RAS is replaced by threonine in RAP proteins. RAP1A induces morphological reversion of a cell line transformed by a Ras oncogene and counteracts the mitogenic function of Ras, since it can interact with Ras GAPs and RAF in a competitive manner. |
| Purity | Greater than 85.0% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | KREV1, G-22K, C21KG, RAP1A, RAP1, Ras-related protein Rap-1A, GTP-binding protein smg p21A, Ras-related protein Krev-1. |
