Description
Data Sheet
| Applications | RAB5A antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 1,000.Recommended starting dilution is 1:500. |
| Clone | P3A4AT. |
| Ig Subclass | Mouse IgG2b heavy chain and light chain. |
| Immunogen | Anti-human RAB5A mAb is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human RAB5A amino acids 1-215 purified from E. coli. |
| Protein Background | RAB5A is a member of RAS oncogene family. The small GTPase, RAB5A is a crucial regulatory component of the endocytic pathway. Activation of RAB5A is mediated by GDP-GTP exchange factors (GEFs) that generate the Rab5-GTP complex). RAB5A is required for the fusion of plasma membranes and early endosomes. |
| Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
| Purification Method | RAB5A antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
| Specifcity | No protease activity detectable, specific activity > 300 U*/mg (*1 U = 1 pmol/min transferred to c-jun (1-169) at 30°C). Autophosphorylation |
| Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. |
| Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Synonyms | Ras-related protein Rab-5A, RAB5A, RAB5. |
| Type | Mouse Anti Human Monoclonal. |
