Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MGSHMTEADV NPKAYPLADA HLTKKLLDLV QQSCNYKQLR KGANEATKTL NRGISEFIVM AADAEPLEII LHLPLLCEDK NVPYVFVRSK QALGRACGVS RPVIACSVTI KEGSQLKQQI QSIQQSIERL LV |
| Description | NHP2L1 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 152 amino acids (1-128a.a.) and having a molecular mass of 16.7kDa.NHP2L1 is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The NHP2L1 protein solution (0.25mg/1ml) is formulated in In 20 mM Tris-HCl Buffer (pH 7.5), 100 mM NaCl, and 10% Glycerol. |
| Protein Background | NHP2L1 is a member of the ribosomal protein L7Ae family. NHP2L1 protein limited to the nucleus, primarily focused in the dense fibrillar component of the nucleolus. NHP2L1 directly binds to the 5′ stem-loop of U4 snRNA and has a vital part in the late stage of spliceosome assembly, prior to step I of splicing catalysis. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Synonyms | FA-1, SPAG12, SNRNP15-5, SNU13, 15.5K, U4/U6.U5 tri-snRNP 15.5 kDa protein, NHP2 Non-Histone Chromosome protein 2-like 1 (S. cerevisiae), Sperm Specific Antigen 1, SSFA1, NHPX, FA1, OTK27, High Mobility Group-like Nuclear Protein 2 homolog 1. |
