Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MSGGLLKALR SDSYVELSQY RDQHFRGDNE EQEKLLKKSC TLYVGNLSFY TTEEQIYELF SKSGDIKKII MGLDKMKKTA CGFCFVEYYS RADAENAMRY INGTRLDDRI IRTDWDAGFK EGRQYGRGRS GGQVRDEYRQ DYDAGRGGYG KLAQNQ |
| Description | NCBP2 produced in E.Coli is a single, non-glycosylated polypeptide chain containing 176 amino acids (1-156a.a.) and having a molecular mass of 20.1kDa.NCBP2 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The NCBP2 protein solution (0.5mg/1ml) is formulated in 20mM Tris-HCl buffer (pH8.0) 0.1M NaCl, 1mM DTT and 20% glycerol. |
| Protein Background | NCBP2 is a part of the nuclear cap-binding protein complex (CBC) that binds to the monomethylated 5” cap of nascent pre-mRNA in the nucleoplasm. NCBP2 protein has an RNP domain usually located in RNA binding proteins, and contains the cap-binding activity. CBC promotes pre-mRNA splicing, 3”-end processing, RNA nuclear export, and nonsense-mediated mRNA decay. |
| Purity | Greater than 95% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Storage | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time.Please avoid freeze thaw cycles. |
| Synonyms | CBP20, NIP1, Cell Proliferation-Inducing Gene 55 protein, NCBP-Interacting Protein 1, Cbc2, CBC2. |
