Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MSLFDKKHLV SPADALPGRN TPMPVATLHA VNGHSMTNVP DGMEIAIFAM GCFWGVERLF WQLPGVYSTA AGYTGGYTPN PTYREVCSGD TGHAEAVRIV YDPSVISYEQ LLQVFWENHD PAQGMRQGND HGTQYRSAIY PLTPEQDAAA RASLERFQAA MLAADDDRHI TTEIANATPF YYAEDDHQQY LHKNPYGYCG IGGIGVCLPP EA. |
| Description | MSRA produced in E.Coli is a single, non-glycosylated polypeptide chain containing 232 amino acids (1-212 a.a.) and having a molecular mass of 25.4kDa.MSRA is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | MSRA protein solution (0.5mg/ml) 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 10% glycerol and 0.1M NaCl. |
| Physical Appearence | Sterile filtered colorless solution. |
| Protein Background | Peptide methionine sulfoxide reductase A (msrA) is an enzyme which catalyzes the reversible oxidation-reduction of methionine sulfoxide in proteins to methionine. MSRA may have a significant function as a repair enzyme for proteins which have been inactivated by oxidation. |
| Purity | Greater than 90.0% as determined by SDS-PAGE. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks.Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | Peptide methionine sulfoxide reductase MsrA, Protein-methionine-S-oxide reductase, Peptide-methionine (S)-S-oxide reductase, Peptide Met(O) reductase, msrA, pms, b4219, JW4178. |
