Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MKLLENSSFE AINSQLTVET GDAHIIGRIE SYSCKMAGDD KHMFKQFCQE GQPHVLEALS PPQTSGLSPS RLSKSQGGEE EGPLSDKCSR KTLFYLIATL NESFRPDYDF STARSHEFSR EPSLSWVVNA VNCSLFSAVR EDFKDLKPQL WNAVDEEICL AECDIYSYNP DLDSDPFGED GSLWSFNYFF YNKRLKRIVF FSCRSISGST YTPSEAGNEL DMELGEEEVE EESRSRGSGA EETSTMEEDR VPVICI |
| Description | MAF1 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 276 amino acids (1-256) and having a molecular mass of 31.0 kDa.The MAF1 is fused to a 20 amino acid His-Tag at N-terminus and purified by proprietary chromatographic techniques. |
| Expression host | E.coli. |
| Formulation | The MAF1 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0) and 10% glycerol. |
| Protein Background | MAF1 resembles Maf1 which is a Saccharomyces cerevisiae a highly conserved protein in eukaryotic cells, localized to the nucleus. MAF1 Interacts with BRF2, MAF1 and functions as a mediator for signals which explicitly inhibit RNA polymerase III (Pol III) activity by inhibiting the association of TFIIIB to DNA. MAF1 reacts to modifications in the cellular environment and represses pol III transcription. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | MAF1 homolog (S. cerevisiae), Repressor of RNA polymerase III transcription MAF1 homolog, homolog of yeast MAF1, MGC20332, MGC31779, MGC39758, DKFZp586G1123. |
