Description
Data Sheet
| Amino acid sequence | MHIHGCDKNH LREIIGILNE VTGEGTPCTE MDVPNVLTAT KNTTESELVCRASKVLRIFY LKHGKTPCLK KNSSVLMELQ RLFRAFRCLD SSISCTMNESKSTSLKDFLE SLKSIMQMDY S. |
| Biological Activity | The ED50 as determined by the dose-dependant induction of HT-2 cell proliferation is less than 2 ng/ml corresponding to a Specific Activity of 500,000IU/mg. |
| Description | Interleukin-4 Mouse Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 120 amino acids and having a molecular mass of 13500 Dalton. The IL-4 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | Lyophilized from a 0.2um concentrated (1mg/ml) solution in PBS pH 7.4. |
| Protein Background | Interleukin-4 is a pleiotropic cytokine produced primarily by activated T lymphocytes, basophils and mast cells. Multiple immune response-modulating functions are performed by IL-4 on a variety of cell types and it has an important role in the regulator of isotype switching, induction of IgE production in B lymphocytes and differentiation of precursor T helper cells. IL-4 binds to both membrane-bound and secreted soluble IL-4 receptors. |
| Purity | Greater than 96.0% as determined by: (a) Analysis by RP-HPLC. (b) Analysis by SDS-PAGE. |
| Quality Control Test | MEGVHRKPSF LALPGHLVKS EETVILQCWS DVMFEHFLLH REGKFNNTLH LIGEHHDGVS KANFSIGPMM PVLAGTYRCY GSVPHSPYQL SAPSDPLDMV IIGLYEKPSL SAQPGPTVQA GENVTLSCSS RSSYDMYHLS REGEAHERRL PAVRSINGTF QADFPLGPAT HGGTYRCFGS FRDAPYEWSN SSDPLLVSVT GN. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| References | Title: CD26, adenosine deaminase, and adenosine receptors mediate costimulatory signalsin the immunological synapse.publication: Published online before printJune 27, 2005, doi:10.1073/pnas.0501050102PNASJuly 5, 2005vol. 102no. 279583-9588.Link: http://www.pnas.org/content/102/27/9583.full.pdf+html |
| Solubility | It is recommended to reconstitute the lyophilized Interleukin 4 in sterile 10mM HAc not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Lyophilized Interleukin-4 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution IL4 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | BCGF, BCDF, B cell stimulating factor, BSF-1, Lymphocyte stimulatory factor 1, IL-4, MGC79402, Binetrakin, Pitrakinra. |
