Description
Data Sheet
| Antibody Properties | Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Description | The HSV-2 gG protein is a synthetic protein which containing the HSV-2 gG immunodominant regions. |
| Formulation | HSV-2 gG solution contains 25mM Tris-HCl pH8.0. |
| Protein Background | Entry of HSV into the host cell involves interactions of several viral glycoproteins with cell surface receptors. The virus particle is covered by an envelope which, when bound to specific receptors on the cell surface, will fuse with the cell membrane and create an opening, or pore, through which the virus enters the host cell. The sequential stages of HSV entry are analagous to those of other viruses. At first, complementary receptors on the virus and cell surface bring the two membranes into proximity. In an intermediate state, the two membranes begin to merge, forming a hemifusion state. Finally, a stable entry pore is formed through which the viral envelope contents are introduced to the host cell. |
| Protein content | By direct ELISA, 1:10,000 dilution will yield >1.0 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Purification Method | HSV-2 gG protein was purified by Prep HPLC. |
| Purity | Protein is >99% pure as determined by Analytical HPLC and Mass Spec. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Storage | HSV-2 gG protein although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
