Description
Data Sheet
| Description | Recombinant GroES produced in E.Coli is a single, non-glycosylated polypeptide chain containing 97 amino acids and having a molecular mass of 10.4 kDa. |
| Expression host | Escherichia Coli. |
| Formulation | The GroES protein (1mg/ml) contains 25mM Tris-HCl buffer (pH 7.5), 100mM NaCl, 5mM DTT and 10% Glycerol. |
| Product Description | Immunoreactive with sera of HCV-infected individuals. |
| Protein Background | GroES protein is the co-chaperonin of GroES in E.coli and assists protein folding. GroEL mediated folding requires the co-chaperonin GroES which is essential for viability. GroES is composed of a single heptameric ring of 10kDa subunits that binds to the ends of the GroEL cylinder. GroES gene was amplified by PCR from E.coli and cloned into an expression vector. This protein was overexpressed in E.coli and was purified by using conventional chromatography techniques. |
| Purity | Greater than 95.0% as determined by analysis by SDS-PAGE. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | CPN10, GROES, HSP10, HSPE1, 10 kDa chaperonin, Protein Cpn10, groES protein, 11.2 kDa stress response protein, Heat shock protein 10. |
