Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MPHIDNDVKL DFKDVLLRPK RSTLKSRSEV DLTRSFSFRN SKQTYSGVPI IAANMDTVGT FEMAKVLCKFSLFTAVHKHY SLVQWQEFAG QNPDCLEHLA ASSGTGSSDF EQLEQILEAI PQVKYICLDV ANGYSEHFVE FVKDVRKRFP QHTIMAGNVV TGEMVEELIL SGADIIKVGI GPGSVCTTRK KTGVGYPQLS AVMECADAAH GLKGHIISDG GCSCPGDVAK AFGAGADFVM LGGMLAGHSESGGELIERDG KKYKLFYGMS SEMAMKKYAG GVAEYRASEG KTVEVPFKGD VEHTIRDILG GIRSTCTYVG AAKLKELSRR TTFIRVTQQV NPIFSEAC. |
| Antibody Properties | Reconstitute with H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Application And Suggested Dilutions | This antibody recognizes both the AD and the AY antigens in direct ELISA and works in capture ELISA with anti-hepatitis Ag-2. |
| Description | GMPR2 Human Recombinant produced in E.Coli is a single, non-glycosylated, polypeptide chain containing 368 amino acids (1-348 a.a.) and having a molecular mass of 40 kDa. GMPR2 is fused to a 20 amino acid His-tag at N-terminus and purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | GMPR2 1mg/ml solution contains 20mM Tris pH-8, 1mM DTT and 10% glycerol. |
| Physical Appearence | Sterile filtered colorless solution. |
| Protein Background | GMPR2 is the single known metabolic step by which guanine nucleotides can be transformed to the pivotal precursor of both adenine and guanine nucleotides. GMPR2 catalyzes the permanent NADPH-dependent reductive deamination of GMP to IMP, and is involved in re-utilization of free intracellular bases and purine nucleosides. |
| Protein content | By direct ELISA (against r.hepatitis vaccine), 1:10,000 dilution will yield 0.5 O.D using alkaline phosphatase conjugated rabbit anti-mouse Ig (Jackson Laboratories). |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Stability | GMPR2 Human Recombinant although stable at 4°C for 1 week, should be stored below -18°C. Please prevent freeze thaw cycles. |
| Synonyms | GMP reductase 2, Guanosine 5”-monophosphate oxidoreductase 2. |
| Titer | Antibody is shipped lyophilized at ambient temperature. |
