Description
Data Sheet
| Amino acid sequence | MQVTVQSSPN FTQHVREQSL VTDQLSRRLI RTYQLYSRTS GKHVQVLANK RINAMAEDGDPFAKLIVETD TFGSRVRVRG AETGLYICMN KKGKLIAKSN GKGKDCVFTE IVLENNYTAL QNAKYEGWYM AFTRKGRPRK GSKTRQHQRE VHFMKRLPRG HHTTEQSLRF EFLNYPPFTR SLRGSQRTWA PEPR. |
| Biological Activity | The specific activity was determined by the dose-dependent stimulation of the proliferation of the Balb/3T3 cell line, the ED50 is 60ng/ml. |
| Description | FGF-8 Human Recombinant produced in HEK cells is a glycosylated monomer, having a molecular weight range of 30-45kDa due to glycosylation.The FGF8 is purified by proprietary chromatographic techniques. |
| Expression host | HEK. |
| Formulation | The FGF-8 was lyophilized in 10mM Tris-HCl pH 7.4 and 800mM NaCl. |
| Physical Appearence | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Protein Background | FGF8 is part of the fibroblast growth factor family. FGF family members have wide mitogenic and cell survival activities, and participate in a variety of biological processes, including embryonic development, cell growth, morphogenesis, tissue repair, tumor growth and invasion. FGF8 supports androgen and anchorage independent growth of mammary tumor cells. FGF8 over expression increases tumor growth and angiogensis. The adult expression of FGF-8 gene is restricted to testes and ovaries. FGF8 functions as an embryonic epithelial factor. FGF8 takes part in midbrain and limb development, organogenesis, embryo gastrulation and left-right axis determination. |
| Purity | Greater than 95% as obsereved by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| Solubility | It is recommended to reconstitute the lyophilized FGF8 in sterile water containing 0.1% endotoxin-free recombinant HSA. |
| Stability | Lyophilized FGF-8 although stable at room temperature for 3 weeks, should be stored desiccated below -18°C. Upon reconstitution FGF8 should be stored at 4°C between 2-7 days and for future use below -18°C.For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Please prevent freeze-thaw cycles. |
| Synonyms | FGF8B, FGF-8B, FGF8-B, KAL6, HBGF-8, HBGF8, AIGF, HBGF-8, MGC149376, fibroblast growth factor 8. |
