Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MGSHMAEQSD EAVKYYTLEE IQKHNHSKST WLILHHKVYD LTKFLEEHPG GEEVLREQAG GDATENFEDV GHSTDAREMS KTFIIGELHP DDRPKLNKPP ETLITTIDSS SS. |
| Description | CYB5A Human Recombinant produced in E.Coli is a single, non-glycosylated polypeptide chain containing 132 amino acids (1-108 a.a) and having a molecular mass of 14.9kDa.CYB5A is fused to a 24 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | CYB5A protein solution (1mg/ml) containing 20mM Tris-HCl buffer (pH8.0), 20% glycerol, 0.1M NaCl and 1mM DTT. |
| link | Purified monoclonal IgG by protein A chromatography. |
| Protein Background | Cytochrome b5 isoform 1 (CYB5A) is a member of the cytochrome b5 family. CYB5A is a membrane bound hemoprotein which functions as an electron carrier for several membrane bound oxygenases. Defects in the CYB5A gene are the cause of methemoglobinemia CYB5A-related (METHB-CYB5A), which is a form of methemoglobinemia (a hematologic disease characterized by the presence of excessive amounts of methemoglobin in blood cells, resulting in decreased oxygen carrying capacity of the blood, cyanosis and hypoxia). |
| Protein concentration | Lateral flow immunoassay. |
| Purity | Greater than 95.0% as determined by SDS-PAGE. |
| Rapid Test Assay | 1. Fresh gold particle prepared within 1 to 2 days with maximal homogeneity in particle size, ? 530 to 534, OD 1.2- 1.4.2. Conjugation pH 8.5, conjugation time should be 6-8 hours.3. Blocking condition: 0.4-0.45% casein. |
| Reagent Appearance | Sterile filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | Cytochrome b5, Microsomal cytochrome b5 type A, MCB5, CYB5A, CYB5. |
