Description
Data Sheet
| Advantages | Starting material tested and certified negative for HIV I & II antibodies, Hepatitis B surface antigen, and Hepatitis C antibodies. |
| Description | Casein Kinase 2 alpha 1 Human Recombinant is a non-glycosylated polypeptide having a molecular mass of 45.1 kDa. Casein Kinase 2 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | CSNK2A1 is supplied in 25mM Tris-HCl, 500mM NaCl, 1mM DTT, 500m PMSF, 50% glycerol, pH 8.5. |
| Protein Background | The Casein kinase 2 (EC2.7.11.1) is a serine/threonine-selective protein kinasethat is a tetramer of two alpha subunits and two beta subunits. The alpha subunits have the catalytic kinase domain. Casein kinase 2 has been implicated in cell cyclecontrol, DNA repair, regulation of the circadian rhythmand other cellular processes. Casein kinase 2 activity has been reported to be activated following Wnt signaling pathwayactivation. A Pertussis toxin-sensitive G proteinand Disheveled appear to be an intermediary between Wnt-mediated activation of the Frizzled receptor and activation of casein kinase 2.Mice that lack casein kinase 2 alpha prime have a defect in the morphology of developing sperm. |
| Purity | Greater than 99% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered clear solution. |
| Storage Conditions | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. Avoid multiple freeze-thaw cycles. |
| Synonyms | Casein kinase II subunit alpha, EC 2.7.11.1, CK II, CK2A1, CKII alpha, CSNK2A1, PKCK2, CSK21. |
| Unit Definition | No protease activity detectable, specific activity > 1.320 U/mg (1U = 1umol/min at 37 degree C) using the synthetic peptide RRRDDDSDDD. |
