Description
Data Sheet
| Applications | Cathepsin H antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:500 ~ 2,000. Recommended starting dilution is 1:1,000. |
| Clone | P3G8AT. |
| Ig Subclass | Mouse IgG2a heavy chain and light chain. |
| Immunogen | Anti-human Cathepsin H mAb, is derived from hybridization of mouse SP2/O myeloma cells with spleen cells from BALB/c mice immunized with recombinant human Cathepsin H amino acids 23-335 purified from E. coli. |
| Protein Background | Cathepsin H is a lysosomal cysteine proteinase important in the overall degradation of lysosomal proteins. Cathepsin H is composed of a dimer of disulfide-linked heavy and light chains, both produced from a single protein precursor. Cathepsin H, which belongs to the peptidase C1 protein family, can act both as an aminopeptidase and as an endopeptidase. Increased expression of the Cathepsin H gene has been associated with malignant progression of prostate tumors. |
| Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
| Purification Method | Cathepsin H antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
| Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. |
| Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Synonyms | Cathepsin H, CTSH, CPSB, ACC-4, ACC-5, MGC1519, minichain, DKFZp686B24257. |
| Type | Mouse Anti Human Monoclonal. |
