Description
Data Sheet
| Antibody Properties | Reconstitute with of H20. Mix gently, wash the sides of the vial and wait 30-60 seconds before use. |
| Applications | CASQ2 antibody has been tested by ELISA and Western blot analysis to assure specificity and reactivity. Since application varies, however, each investigation should be titrated by the reagent to obtain optimal results. Recommended dilution range for Western blot analysis is 1:1000.Recommended starting dilution is 1:1000. |
| Available Conjugates | Blocking and staining antibody. For staining, use 10ul/1,000,000 cells. Titer for blocking T cell activation should be determined by the investigator. |
| Clone | PAT4E10AT. |
| Ig Subclass | Mouse IgG1 heavy chain and k light chain. |
| Immunogen | Anti-human CASQ2 mAb, is derived from hybridization of mouse FO myeloma cells with spleen cells from BALB/c mice immunized with recombinant human CASQ2 amino acids 20-399 purified from E. coli. |
| Immunological function | This antibody is only available non-conjugated to Biotin. |
| Protein Background | CASQ2 belongs to the calsequestrin family and is localized to the sarcoplasmic reticulum in cardiac and slow skeletal muscle cells. CASQ2 is a calcium binding protein that stores calcium for muscle function. The discharge of calcium bound to CASQ2 through a calcium release channel activates muscle contraction. CASQ2 binds 40 to 50 moles of calcium. CASQ2 mutations result in stress-induced polymorphic ventricular tachycardia, also called catecholaminergic polymorphic ventricular tachycardia 2 which is known fir its bidirectional ventricular tachycardia that causes cardiac arrest. |
| Protein formulation | 1mg/ml containing PBS, pH-7.4, & 0.1% Sodium Azide. |
| Purification Method | CASQ2 antibody was purified from mouse ascitic fluids by protein-G affinity chromatography. |
| Reconstitution | 1mg/ml in PBS (after reconstitution). |
| Stability / Shelf Life | 12 months at -20°C. 1 month at 4°C. |
| Storage Procedures | For periods up to 1 month store at 4°C, for longer periods of time, store at -20°C. Prevent freeze thaw cycles. |
| Synonyms | PDIB2, CASQ2, Calsequestrin-2, Calsequestrin cardiac muscle isoform, FLJ26321, FLJ93514. |
| Type | Mouse Anti Human Monoclonal. |
