Description
Data Sheet
| Description | Recombinant Rat Carboxypeptidase-B is expressed in E.Coli having a Mw of 35kDa is purified by standard chromatography techniques. Recombinant Rat Carboxypeptidase-B is free from foreign enzymes such as carboxypeptidase A & chymotrypsin. Recombinant Carboxypeptidase-B is free from protease inhibitors such as PMSF and EDTA. |
| Expression host | Escherichia Coli. |
| Formulation | The protein was lyophilized with 100mM NaCl and 20mM Tris pH-7.5. |
| Protein Background | Carboxypeptidase B (EC 3.4.17.2) catalyzes hydrolysis of the basic amino acids lysine, arginine and ornithine from the C-terminal end of polypeptides. The Mw was found to be 34.5 kda, optimun pH-7.9, and pI-6. Carboxypeptidase B is inhibited by arginine, lysine and ornithine. The enzyme is not inhibited by di-isopropylfluorophosphate (DFP), but it is inhibited by metal chelating agents, e.g., EDTA, 1,10-phenanthroline. |
| Purity | Greater than 90% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered lyophilized powder. |
| Solubility | It is recommended to reconstitute the lyophilized Rat Carboxypeptidase-B in sterile 18M-cm H2O not less than 100ug/ml, which can then be further diluted to other aqueous solutions. |
| Stability | Recombinant Rat Carboxypeptidase-B although stable at room temp for 1 week, should be stored desiccated below -18°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles. |
| Synonyms | Carboxypeptidase B, Cpb1, Cpb. |
| Unit Definition | One Unit hydrolyzes one micromole of hippuryl-L-arginine per minute at 25°C, pH-7.65. |
