Description
Data Sheet
| Amino acid sequence | MGSSHHHHHH SSGLVPRGSH MSDLRITEAF LYMDYLCFRA LCCKGPPPAR PEYDLVCIGL TGSGKTSLLS KLCSESPDNV VSTTGFSIKA VPFQNAILNV KELGGADNIR KYWSRYYQGS QGVIFVLDSA SSEDDLEAAR NELHSALQHP QLCTLPFLIL ANHQDKPAAR SVQEIKKYFE LEPLARGKRW ILQPCSLDDM DALKDSFSQL INLLEEKDHE AVRM |
| Application | 1. Avidin-Biotin does not exhibit unwanted non-specific binding that have been previously reported for the native egg white avidin.2. The non-specific properties of Avidin-Biotin have been tested in ELISA, Western Blot & Immunohistochemistry techniques and have been found to be superior to unmodified avidin. 3. Avidin-Biotin rarely affected by pH values between the range of pH-2 to pH-12 or by guanidine-HCl concentrations up to 8M at neutral pH. |
| Description | ARL15 Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 224 amino acids (1-204) and having a molecular mass of 25.0 kDa.ARL15 is fused to a 20 amino acid His-tag at N-terminus & purified by proprietary chromatographic techniques. |
| Expression host | E.coli. |
| Formulation | The ARL15 solution (1mg/ml) contains 20mM Tris-HCl buffer (pH 8.0), 1mM DTT, 0.1M NaCl and 10% glycerol. |
| Protein Background | ARL15 is a member of the ARF family which are essential in eukaryotic vesicular trafficking pathways and have a vital part in the activation of phospholipase D. ARL15 variants influence levels of Acrp30, an adipocyte-derived protein that is extremely genetic and inversely related to the prospect of type 2 diabetes mellitus and coronary heart disease. |
| Purity | Greater than 85% as determined by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered colorless solution. |
| Stability | Store at 4°C if entire vial will be used within 2-4 weeks. Store, frozen at -20°C for longer periods of time. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA).Avoid multiple freeze-thaw cycles. |
| Synonyms | ADP-ribosylation factor-like 15, ADP-ribosylation factor related protein 2, ARFRP2, ARF-related protein 2, FLJ20051. |
