Description
Data Sheet
| Advantages | Plasma from each donor has been tested and found negative for antibody to HIV-1, HIV-2, HCV, HBSAG and Syphilis. |
| Amino acid sequence | VYLSECKTGN GKNYRGTMSK TKNGITCQKW SSTSPHRPRF SPATHPSEGL EENYCRNPDN DPQGPWCYTT DPEKRYDYCD ILECEEECMH CSGENYDGKI SKTMSGLECQ AWDSQSPHAH GYIPSKFPNK NLKKNYCRNP DRELRPWCFT TDPNKRWELC DIPRCTTPPP SSGPTYQCLKGTGENYRGNV AVTVSGHTCQ HWSAQTPHTH NRTPENFPCK NLDENYCRNP DGKRAPWCHT TNSQVRWEYC KIPSCDSSP. |
| Biological Activity | The activity is assayed on anti-proliferation and anti-migration of endothelial cells in vitro and anti-angiogenesis in vivo. The specific activity of anti-migration of endothelial cells in vitro is 55,000 Units/mg. |
| Description | Angiostatin Human Recombinant produced in E.coli is a single, non-glycosylated polypeptide chain containing 259 amino acids and having a molecular mass of approximately 30.0 kDa. The Ang K1-3 is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia coli. |
| Formulation | Lyophilized from a 0.2m filtered concentrated (1.0mg/ml) solution in 20mM NaAc, pH5.5, 4% mannitol. |
| Human Virus Test | The Iron content was estimated by ICP and was found to be 2.0 ppm. |
| Protein Background | Ang K1-3 is a proteolytic fragment of plasminogen containing the first three kringle structures. A specific inhibitor of endothelial cell growth and angiogenesis. More active relative to kringles 1-4. Ang K1-3 reduces endothelial cell proliferation and acts as a potent inhibitor of angiogenesis and tumor growth. |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile Filtered White lyophilized (freeze-dried) powder. |
| References | Title: Regenerative protein thymosin b-4 is a novel regulator of purinergic signaling. Publication: Published online before printNovember 24, 2010, doi:10.1096/fj.10-169417 March 2011The FASEB Journalvol. 25no. 3907-915.Link: http://www.fasebj.org/content/25/3/907.full.pdf+html |
| Solubility | We recommend to briefly centrifuge the vial prior to opening to bring the contents to the bottom. Reconstitute in sterile distilled water or aqueous buffer containing 0.1% BSA to a concentration of 0.1-1.0 mg/ml. Stock solutions should be apportioned into working aliquots and stored at |
| Stability | The lyophilized Angiostatin K1-3 is stable for several weeks at 2-8°C, but should be kept at -20°C for long term storage, preferably desiccated. Upon reconstitution, the preparation is stable for up to one week at 2-8°C. For maximal stability, apportion the reconstituted preparation into working aliquots and store at -20°C to -70°C. Avoid repeated freeze/thaw cycles. |
| Synonyms | Angiostatin, Angiostatin Kringles 1-3, Ang K1-3. |
