Description
|
Assay Range |
15.6 – 1,000 pg/mL |
|
Sensitivity |
1.0 pg/mL |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Rat TNF alpha standard: 2 vials
2. One 96-well plate coated with Rat TNF alpha Ab
3. Sample diluent buffer: 12 ml— 2
4. Detection antibody: 130 µL, dilution 1:100
5. Streptavidin-HRP: 130 µL, dilution 1:100
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. TMB developing agent: 10 mL x1
9. Stop solution: 10 mL x1
10. Washing solution (20x): 25 mL x1
PRODUCT CITATION:
1. Effect of arsenic exposure on MDA, SOD, H2O2 and TNF-α levels of uterus homogenate of female Sparague-Dawleys rats: Irnawati Irnawati et al.
F1000 Research 2023, 12:108 Last updated: 23 MAY 2023
