Description
Sensitivity
10 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant Mouse CD62L standard: 2 ng/vial – 2.
2. One 96-well plate precoated with anti-Mouse CD62L Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Mouse CD62L Ab: 1 vial, dilution 1:500.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
L-selectin, also known as CD62L, is a cell surface component that is a member of a family of adhesion/homing receptors that play important roles in lymphocyte-endothelial cell interactions. It is expressed in B-cell lines and T-lymphocytes and belongs to the selectin family of proteins, which recognize sialylated carbohydrate groups.CD62L is composed of multiple domains: one homologous to lectins, one to epidermal growth factor, and two to the consensus repeat units found in C3/C4-binding proteins. CD62L mediates the adherence of lymphocytes to endothelial cells of high endothelial venules in peripheral lymph nodes and promotes initial tethering and rolling of leukocytes in endothelia.
Data Sheet
| Assay Range | 12.5 – 800 pg/ml |
