Description
Data Sheet
| Alternative Name of Analyte | CCN3；NOVh；IBP-9；IGFBP9；IGFBP-9 |
| Assay duration | 4 hours |
| Assay format | Sandwich |
| Assay principle | This Mouse NOV ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse NOV specific antibodies were precoated onto 96-well plates. The Mouse NOV specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse NOV amount of sample captured in plate. |
| Assay Range | 93.75-6000 pg/ml |
| Background | The protein encoded by this gene is a small secreted cysteine-rich protein and a member of the CCN family of regulatory proteins. CNN family proteins associate with the extracellular matrix and play an important role in cardiovascular and skeletal development, fibrosis and cancer development. |
| Sample type | serum, plasma, cell culture supernatant |
| Sensitivity | 9 pg/ml |
| Size | 96 Tests |
| Specificity | reactive with Mouse NOV, reactivity with other related proteins not observed |
| Storage | 2-8℃ |
