Description
Data Sheet
| Alternative Name of Analyte | MFGE8；BA46；EDIL1；HMFG；HsT19888；MFG-E8；MFGM；OAcGD3S；SED1；SPAG10；hP47 |
| Assay duration | 4 hours |
| Assay format | Sandwich |
| Assay principle | This Mouse MFG-E8 ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Mouse MFG-E8 specific antibodies were precoated onto 96-well plates. The Mouse MFG-E8 specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Mouse MFG-E8 amount of sample captured in plate. |
| Assay Range | 31.25-2000 pg/ml |
| Background | Milk fat globule-EGF factor 8 protein Mfge8), also known as lactadherin, is a protein which is encoded by the MFGE8 gene. |
| Sample type | serum, plasma, cell culture supernatant |
| Sensitivity | 3 pg/ml |
| Size | 96 Tests |
| Specificity | reactive with Mouse MFG-E8, reactivity with other related proteins not observed |
| Storage | 2-8℃ |
