Description
CD16 is a low affinity Fc receptor. In human, CD16 is expressed as two distinct forms (CD16a and CD16b) encoded by two different highly homologous genes in a cell type-specific manner. CD16b is a low-affinity, GPI-linked receptor expressed by neutrophils and eosinophils, whereas CD16a is an intermediate affinity polypeptide-anchored transmembrane glycoprotein expressed by a subset of T lymphocytes, natural killer (NK) cells, monocytes, and macrophages. The receptors bind to the Fc portion of IgG antibodies which then activates the NK cell for Antibody-Dependent Cell-mediated Cytotoxicity (ADCC). A lack of CD16 in a given population of neutrophils may indicate prematurity, as could be caused by a left-shift due to neutrophilic leukocytosis induced by tissue necrosis or bacterial infection.
Data Sheet
| Application | For quantitative determination of Cynomolgus CD16 /FCGR3. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Assay Range | 12.5 – 800 pg/ml |
| Expiration | Six months at 2 – 8 ⁰C. |
| Sample type | Culture Supernates, Serum, plasma, body fluids, tissue lysates. |
| Sensitivity | 10 pg/ml |
| Size | 96T |
| Storage | Store at 2 – 8⁰C. Keep Standard and detection antibody at -20⁰C. |
