Description
Sensitivity
30 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human PDGFRA standard: 2 ng/vial – 2.
2. One 96-well plate precoated with anti-human PDGFRA Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human PDGFRA Ab: 1 vial, dilution 1:800.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
DGFRA (Alpha-type platelet-derived growth factor receptor) is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides.
Data Sheet
| Assay Range | 31.25 – 2000 pg/ml |
