Description
Sensitivity
5 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human JAM-A standard: 1ng/vial – 2.
2. One 96-well plate precoated with anti-human JAM-A Ab
3. Sample diluent buffer: 12 mL x 1
4. Biotin-conjugated anti-Human JAM-A Ab: 1 vial.
5. Detection Antibody diluent buffer: 12 mL x 2
6. Streptavidin-HRP: 1 vial, 1:200 dilution.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Human JAM-A, also known as platelet adhesion molecule 1 (PAM-1), platelet F11 receptor and CD321, is the firstly identified member of the Junctional Adhesion Molecule (JAM) family comprising at least three members. CD321 is a multifunctional protein that is primarily expressed by platelets, endothelial and epithelial cells. The CD321 protein co-localizes with tight junction molecules in both epithelial and endothelial cells and plays an important role in the regulation of junctional integrity and permeability.
Data Sheet
| Assay Range | 7.8 – 500 pg/ml |
