Description
Data Sheet
| Alternative Name of Analyte | Hepatocyte Growth Factor Receptor；c-MET；MET；HGFR；AUTS9；RCCP2 |
| Assay duration | 4 hours |
| Assay format | Sandwich |
| Assay principle | This Human HGF R/c-MET ELISA Kit was based on standard sandwich enzyme-linked immunosorbent assay technology. Human HGF R/c-MET specific antibodies were precoated onto 96-well plates. The Human HGF R/c-MET specific detection antibodies were biotinylated. The test samples and biotinylated detection antibodies were added to the wells subsequently and then followed by washing the plate. Streptavidin-HRP was added and unbound conjugates were washed away with wash buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow color is proportional to the Human HGF R/c-MET amount of sample captured in plate. |
| Assay Range | 62.5-4000pg/ml |
| Background | A product of the hepatocyte growth factor receptor proto-oncogene c-Met is a heterodimeric transmembrane glycoprotein, is a receptor tyrosine kinase. c-MET synthesis of single-chain precursor (PR170) post-translational glycosylation, proteolytic cleavage to produce the mature form of the heterodimer. |
| Sample type | serum；plasma，cell culture supernatant |
| Sensitivity | 14 pg/ml |
| Size | 96 Tests |
| Specificity | reactive with Human HGF R/c-MET，reactivity with other related proteins not observed |
| Storage | 2-8℃ |
