Description
|
Assay Range |
156.2-10,000 pg/mL |
|
Sensitivity |
50 pg/mL |
|
Specificity |
No cross-reaction with other related substances detected |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples |
|
Sample Type |
Cell lysates |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Human HAI-1 standard: 2 vials
2. One 96-well plate coated with Human HAI-1 Ab
3. Sample diluent buffer: 12 mL – 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9.Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Background
HAI-1 is a Kunitz-type serine protease inhibitor and functions as a strong inhibitor of HGF activator (HGFA) and matriptase. HAI-1 is an integral membrane protein consisting of two Kunitz domains, a LDL receptor-like domain, and a C-terminal transmembrane domain. Two soluble forms are generated by ectodomain shedding, one with a single Kunitz domain and the other with two Kunitz domains. HAI-1 is also a specific receptor of active HGFA, acting as a reservoir of this enzyme on the cell surface. The shedding of HAI-1 and HGFA/HAI-1 complex is enhanced by treatment with phorbol 12 myristate 13 acetate or IL1β.
