Description
Sensitivity
4 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD95 standard: 1 ng/vial – 2.
2. One 96-well plate precoated with anti-human CD95 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-HumanCD95 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD95, also known as APO-1, Fas and TNFRSF6, is a cell surface glycoprotein expressed by a substantial minority of resting T and B cells, and by about 5% of resting NK cells. CD95 is the receptor for TNFSF6/FASLG. The adapter molecule FADD recruits caspase-8 to the activated receptor. The resulting death-inducing signaling complex (DISC) performs caspase-8 proteolytic activation which initiates the subsequent cascade of caspases, mediating apoptosis. CD95 is strongly upregulated on activated T, B and NK cells.Defects in FAS are the cause of autoimmune lymphoproliferative syndrome type 1A (ALPS1A).
Data Sheet
| Assay Range | 4.7- 300 pg/ml |
