Description
Sensitivity
150 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD84 standard: 10 ng/vial – 2.
2. One 96-well plate precoated with anti-human CD84 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD84 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD84 is a member of the CD2 subset of the Ig superfamily of cell surface molecules. It is preferentially expressed on B lymphocytes, monocytes, and platelets. CD84 was positive on CD4-CD8- thymocytes, and its expression decreased with cell maturation. It is expressed on mature T cells preferentially on CD45RO+.
Data Sheet
| Assay Range | 156 – 10000 pg/ml |
