Description
Sensitivity
50pg/mL
Size
96T
Assay Principle
Sandwich ELISA
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant human CA14 standard: 10ng/vial – 2.
2. One 96-well plate precoated with anti-human CA14 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CA14 Ab: 1 vial, dilution 1:2000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. Assay diluent buffer: 12 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Carbonic anhydrase 14 is an enzyme that in humans is encoded by the CA14 gene.Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. CA XIV is predicted to be a type I membrane protein and shares highest sequence similarity with the other transmembrane CA isoform, CA XII; however, they have different patterns of tissue-specific expression and thus may play different physiologic roles. In melanocytic cells CA14 gene expression may be regulated by MITF.
Data Sheet
| Assay Range | 125 – 8000 pg/ml |
