Description
Sample Volume
100 µL final volume, dilution factor varies on samples
Detection Method
Chromogenic
Kit Components
1. Recombinant B4GALT1 standard: 10ng/vial – 2.
2. One 96-well plate precoated with anti-B4GALT1 antibody.
3. Sample diluent buffer: 12 ml
4. HRP conjugated anti-B4GALT1 antibody: 1 vial, dilution 1:1000.
5. Detection Antibody diluention buffer: 12ml.
6. Assay diluent buffer: 12 ml.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
β-1,4-Galactosyltransferase 1 (B4GALT1) is one of seven B4GALT genes. Human B4GALT1 encodes a type II membrane protein, which is ubiquitously expressed and involved in glycoconjugate biosynthesis. A soluble form (aa 78 to 398), derived from proteolytic cleavage, is a component of lactose synthase in milk that also contains α-lactalbumin. Defects in B4GALT1 are the cause of multisystem disorders present with a wide variety of clinical features, such as disorders of the nervous system development, psychomotor retardation, dysmorphic features, hypotonia, coagulation disorders, and immunodeficiency.
Data Sheet
| Application | For quantitative determination of human Beta-1,4-galactosyltransferase 1. |
| Assay principle | This assay is a sandwich Enzyme Linked-Immuno-Sorbent Assay (ELISA). |
| Assay Range | 156 – 10000 pg/ml |
| Expiration | Six months at 2 – 8 ⁰C. |
| Sample type | Serum, plasma, body fluids, tissue lysates. |
| Sensitivity | 100 pg/ml |
| Size | 96T |
| Storage | Store at 2 – 8⁰C. Keep Standard and detection antibody at -20⁰C. |
