Description
|
Assay Range |
156.25-10,000 pg/mL |
|
Sensitivity |
50 pg/mL |
|
Specificity |
No cross-reaction with other related substances detected |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples |
|
Sample Type |
Cell culture supernatants |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Bovine IL-2 standard: 2 vials
2. One 96-well plate coated with Bovine IL-2 Ab
3. Sample diluent buffer: 12 mL – 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer: 12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9. Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Background
Interleukin 2 (IL-2), also known as T cell growth factor (TCGF), is a member of the common gamma chain (γc) cytokine family. The 153 amino acid (aa) human IL-2 precursor contains a 20 aa signal sequence and a 133 aa mature region with one O-linked glycosylation site at Thr3, plus three cysteines, two of which form an intrachain disulfide bond that is essential for IL-2 bioactivity. Human IL-2 shares approximately 60% sequence identity with mouse IL-2, and is functional on mouse IL-2 responsive cells. IL-2 is expressed and secreted on γδT cells, activated conventional CD4+ and CD8+ T cells, neurons, microglia, and hematopoietic stem cells in humans.
IL-2 first binds to IL-2 receptorα (IL-2Rα)/ CD25 in the binary receptor complex then recruits IL-2Rβ/CD122 and IL-2Rγ/CD132 to trigger a variety of biological processes. IL-2 plays a key role in the development, maintenance and function of regulatory T cells and in promoting the development of activated CD8+ T cells into memory cells.
