Description
|
Assay Range |
15.6-1000 pg/mL |
|
Sensitivity |
1.0 pg/mL |
|
Specificity |
No cross-reaction with other related substances detected |
|
Size |
96T |
|
Storage |
Store at 2 – 8ºC. Keep reconstituted standard and detection Ab at -20 ºC |
|
Assay Principle |
Sandwich ELISA |
|
Sample Volume |
100 µL final volume, dilution factor varies on samples |
|
Sample Type |
Cell culture supernatants, serum and plasma. |
|
Detection Method |
Chromogenic |
Kit Components
1. Recombinant Canine CCL2/MCP-1 standard: 2 vials
2. One 96-well plate coated with Canine CCL2/MCP-1 Ab
3. Sample diluent buffer: 12 mL – 1
4. Detection antibody: 1 vial
5. Streptavidin-HRP: 1 vial
6. Antibody diluent buffer:12 mL x1
7. Streptavidin-HRP diluent buffer: 12 mL x1
8. Chromogenic solution A: 6 mlx1
9. Chromogenic solution B: 6 mlx1
10. Stop solution: 6 mL x1
11. Washing solution (20x): 25 mL x1
Background
Monocyte Chemotactic Protein-1 (MCP-1), also known as CCL2, MCAF and TDCF, is a heparin-binding small protein belonging to the C-C motif chemokine family. Human MCP-1 cDNA encodes a 99 amino acid (aa) precursor consisting of a 23 aa signal peptide and a 76 aa mature protein with a receptor binding and dimerization N-terminus and a glycosaminoglycan (GAG)-binding C-terminus. Mature human MCP-1 is 57% aa identical to the mouse ortholog (JE). Multiple proteolytically processed isoforms of MCP-1, such as the N-terminally truncated forms of 71 aa and 72 aa residues, as well as the c-terminally truncated form of 69 aa residues. MCP-1 is secreted by many different cell types, including endothelial cells (EC), monocytes, fibroblasts, vascular smooth muscle cells, mast cells, and astrocytes.
The MCP-1 receptors are three G-protein-coupled receptors: CCR2, CCR4 and D6/CCBP2. Primarily, MCP-1 plays a chemotactic role in monocytes and basophils. Clinically, MCP-1 has been implicated in pathogeneses of several diseases characterized by monocytic infiltrates, such as psoriasis, rheumatoid arthritis and atherosclerosis.
