Description
Background
CHEK2(checkpoint kinase 2), also called CHK2 or RAD53, is a cell cycle checkpoint regulator and putative tumor suppressor which contains a forkhead-associated protein interaction domain essential for activation in response to DNA damage and is rapidly phosphorylated in response to replication blocks and DNA damage. CHEK2 is mapped on 22q12.1. CHEK2 was modified by phosphorylation and activated in response to ionizing radiation, and was also modified in response to hydroxyurea treatment. In response to DNA damage, CHEK2 stabilizes the p53 tumor suppressor protein leading to cell cycle arrest in G1. Oligomerization of CHEK2 increases the efficiency of transautophosphorylation, resulting in the release of active CHEK2 monomers that proceed to enforce checkpoint control in irradiated cells. Following gamma irradiation, CHEK2 phosphorylated PML on ser117, causing dissociation of the 2 proteins.
Data Sheet
| Form | lyophilized |
| Ig type | rabbit IgG |
| Immunogen/Antigen | A synthetic peptide corresponding to a sequence at the N-terminal |
| Reconstitution | 0.2ml of distilled water will yield a concentration of 500μg/ml. |
| Size | 100ug/vial |
| Storage | At -20C for one year. After reconstitution, at 4C for one month. |
