Description
AAT1, ALAT, ALT, Glutamic-alanine transaminase 1, Glutamic-pyruvic transaminase 1, GPT 1, SGPT, ALT1
Data Sheet
| Biological Activity | The specific activity was found to be 1,020 U/mg. |
| Description | Alanine Aminotransferase Human Recombinant produced in E.Coli is a homodimer, non-glycosylated, polypeptide chain containing 495a.a and having a molecular mass of 54,479 Dalton. The amino acid sequence is the same as that of native form of human liver ALT.The ALT is purified by proprietary chromatographic techniques. |
| Expression host | Escherichia Coli. |
| Formulation | The protein was dialyzed against 40mM sodium acetate buffer (pH 5.5), 1mM DTT,1mM EDTA, 5mM 2-oxoglutarate and 0.1mM pyridoxal-5′-phosphate. |
| Product Description | Immunoreactive with sera of HCV-infected individuals. |
| Protein Background | Alanine transaminase or ALT is a transaminaseenzyme.ALT is found in serumand in various bodily tissues, but is most commonly associated with the liver; It catalyzes the transfer of an aminogroup from alanineto a-ketoglutarate, the products of this reversible transaminationreaction being pyruvateand glutamatealanine+ a-ketoglutarate= pyruvate+ glutamateIt is commonly measured clinically as a part of a diagnostic liver function test, to determine liver health. It is also called serum glutamate pyruvate transaminase (SGPT) or alanine aminotransferase (ALAT). Diagnostically, it is almost always measured in units/litre (U/L). |
| Purity | Greater than 95.0% as determined by:(a) Analysis by RP-HPLC.(b) Analysis by SDS-PAGE. |
| Reagent Appearance | Sterile liquid formulation. |
| Stability | AAT1 although stable at 10°C for 5 days, should be stored below -18°C.Please prevent freeze-thaw cycles. |
| Synonyms | Alanine aminotransferase 1, ALT1, EC 2.6.1.2, Glutamate pyruvate transaminase 1, GPT 1, Glutamic–alanine transaminase 1, Glutamic–pyruvic transaminase 1, GPT, AAT1, GPT1. |
