Human Dengue IgM Antibody ELISA Kit

Human Dengue fever IgG ELISA kit

NG-E10001

Human Dengue fever IgM ELISA kit

NG-E10002

Human Dengue Virus IgM µ-capture ELISA Kit

NN-E10001

Human Dengue Fever IgG/IgM ELISA Kit

NR-R10181

Dengue virus NS1 antigen ELISA kit

NR-R10004

Zika Virus NS1 Antigen ELISA Kit

NB-E400926

Zika Virus Envelope (Env) Antigen ELISA Kit

NB-E400927

Human Zika Virus IgG ELISA Kit

NB-400926G

Human Zika Virus IgM µ-capture ELISA Kit

NB-400926M

Microplates are coated with specific antigens to bind corresponding antibodies of the sample. After washing the wells to remove all unbound sample material a horseradish peroxidase (HRP) labelled conjugate is added. This conjugate binds to the captured antibodies. In a second washing step unbound conjugate is removed. The immune complex formed by the bound conjugate is visualized by adding Tetramethylbenzidine (TMB) substrate which gives a blue reaction product.
The intensity of this product is proportional to the amount of specific antibodies in the sample. Sulphuric acid is added to stop the reaction. This produces a yellow endpoint colour. Absorbance at 450/620 nm is read using an ELISA microwell plate reader.