Description
Sensitivity
90 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human CD160 standard:10 ng/vial – 2.
2. One 96-well plate precoated with anti-human CD160 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human CD160 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
CD160 antigen, also known as Natural killer cell receptor BY55 and CD160, is a GPI-anchored cell membrane glycoprotein with one Ig-like V-type domain. CD160 is expressed in spleen, peripheral blood, and small intestine. Expression is restricted to functional NK and T cytotoxic lymphocytes. On a subpopulation of cytolytic T cells and NK cells, CD160 functions as a broad specificity receptor for MHC class I and related molecules. When expressed on vascular endothelial cells, CD160 propagates anti-angiogenic signals and promotes apoptosis.
Data Sheet
| Assay Range | 93.7 – 6000 pg/ml |
