Description
Sensitivity
40 pg/mL
Size
96T
Storage
Store at 2 – 8ºC. Keep Standard and detection antibody at -20 ºC
Assay Principle
Sandwich ELISA
Sample volume
100 µL final volume, dilution factor varies on samples.
Detection Method
Chromogenic
Kit Components
1. Recombinant human P53R2 standard:5 ng/vial – 2.
2. One 96-well plate precoated with anti-human P53R2 Ab
3. Sample diluent buffer: 12 mL x 1
4. HRP conjugated anti-Human P53R2 Ab: 1 vial, dilution 1:1000.
5. Detection Antibody diluent buffer: 12 mL x 1
6. 20 x Wash Solution: 25 ml x 1.
7. TMB color developing agent A: 6 ml.
8. TMB color developing agent B: 6 ml.
9. TMB stop solution: 6ml.
Background
Ribonucleoside reductase subunit M2B (also known as RRM2B or P53R2) is an enzyme belonging to the iron- dependent ribonucleotide reductase (RNR) enzyme family, which is essential for DNA synthesis. P53R2 is widely expressed at a high level in skeletal muscle and at a weak level in thymus. It also expresses in epithelial dysplasias and squamous cell carcinoma. P53R2 translocates from cytoplasm to nucleus in response to DNA damage. This protein plays a pivotal role in cell survival by repairing damaged DNA in a p53/TP53-dependent manner and supplies deoxyribonucleotides for DNA repair in cells arrested at G1 or G2.P53R2 defect has been shown to cause progressive external ophthalmoplegia.
Data Sheet
| Assay Range | 47 – 3000 pg/ml |
