Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| plasminogen activator, tissue | Polyclonal | IgG | Rabbit | Human, Mouse | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human TPA recombinant protein (Position: H366-P562). Human TPA shares 83% and 84% amino acid (aa) sequence identity with mouse and rat TPA, respectively. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
PLAT |
Protein |
Tissue-type plasminogen activator |
Uniprot ID |
P00750 |
Function |
Converts the abundant, but inactive, zymogen plasminogen to plasmin by hydrolyzing a single Arg-Val bond in plasminogen. By controlling plasmin-mediated proteolysis, it plays an important role in tissue remodeling and degradation, in cell migration and many other physiopathological events. Plays a direct role in facilitating neuronal migration. |
Tissue Specificity |
Synthesized in numerous tissues (including tumors) and secreted into most extracellular body fluids, such as plasma, uterine fluid, saliva, gingival crevicular fluid, tears, seminal fluid, and milk. |
Sub-cellular localization |
Secreted, extracellular space. |
Sequence Similarities |
Belongs to the peptidase S1 family. |
Aliases |
Alteplase antibody|DKFZp686I03148 antibody|Plasminogen activator tissue antibody|Plasminogen activator tissue type antibody|Plasminogen Activator Tissue Type Isoform 1 Preproprotein antibody|PLAT antibody|Reteplase antibody|t PA antibody|T Plasminogen Activator antibody|t-PA antibody|T-plasminogen activator antibody|Tissue plasminogen activator (t PA) antibody|Tissue type plasminogen activator antibody|Tissue-type plasminogen activator chain B antibody|TPA antibody|TPA_HUMAN antibody|TPA1 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human, Mouse | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TPA antibody, ASA-B1899, Western blotting
All lanes: Anti TPA (ASA-B1899) at 0.5ug/ml
Lane 1: Mouse Lung Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: A375 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 85KD
Observed bind size: 85KD
All lanes: Anti TPA (ASA-B1899) at 0.5ug/ml
Lane 1: Mouse Lung Tissue Lysate at 50ug
Lane 2: Mouse Testis Tissue Lysate at 50ug
Lane 3: U87 Whole Cell Lysate at 40ug
Lane 4: A431 Whole Cell Lysate at 40ug
Lane 5: A375 Whole Cell Lysate at 40ug
Lane 6: A549 Whole Cell Lysate at 40ug
Predicted bind size: 85KD
Observed bind size: 85KD
