Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| translocation associated membrane protein 2 | Polyclonal | IgG | Rabbit | Human, Mouse, Rat | WB | Immunogen affinity purified. |
Immunogen |
||||||
| A synthetic peptide corresponding to a sequence at the C-terminus of mouse TRAM2(309-325aa RFIHSQLRHWREYWKEQ), identical to the related rat sequence, and different from the related human sequence by one amino acid. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg Thimerosal and 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TRAM2 |
Protein |
Translocating chain-associated membrane protein 2 |
Uniprot ID |
Q924Z5 |
Function |
Necessary for collagen type I synthesis. May couple the activity of the ER Ca(2+) pump SERCA2B with the activity of the translocon. This coupling may increase the local Ca(2+) concentration at the site of collagen synthesis, and a high Ca(2+) concentration may be necessary for the function of molecular chaperones involved in collagen folding (By similarity). |
Tissue Specificity |
|
Sub-cellular localization |
Membrane ; Multi-pass membrane protein . |
Sequence Similarities |
Belongs to the TRAM family. |
Aliases |
C330003D03Rik antibody|KIAA0057 antibody|MGC25725 antibody|OTTHUMP00000016607 antibody|TRAM like protein antibody|Tram2 antibody|TRAM2_HUMAN antibody|Translocating chain associating membrane protein 2 antibody|Translocating chain-associated membrane protein 2 antibody|Translocation associated membrane protein 2 antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Mouse Human, Rat | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti-TRAM2 antibody, ASA-B1911, Western blotting
Recombinant Protein Detection Source: E.coli derived -recombinant Mouse TRAM2,39.0KD(162aa tag+R190-P370)
Lane 1: Recombinant Mouse TRAM2 Protein 10ng
Lane 2: Recombinant Mouse TRAM2 Protein 5ng
Lane 3: Recombinant Mouse TRAM2 Protein 2.5ng
Recombinant Protein Detection Source: E.coli derived -recombinant Mouse TRAM2,39.0KD(162aa tag+R190-P370)
Lane 1: Recombinant Mouse TRAM2 Protein 10ng
Lane 2: Recombinant Mouse TRAM2 Protein 5ng
Lane 3: Recombinant Mouse TRAM2 Protein 2.5ng
