Description
Overview
Long Name |
Antibody Type |
Antibody Isotype |
Host |
Species Reactivity |
Validated Applications |
Purification |
| transient receptor potential cation channel, subfamily C, member 5 | Polyclonal | IgG | Rabbit | Human | WB | Immunogen affinity purified. |
Immunogen |
||||||
| E.coli-derived human TRPC5 recombinant protein (Position: K684-L973). Human TRPC5 shares 90% amino acid (aa) sequence identity with mouse TRPC5. | ||||||
Properties
Form |
Lyophilized |
Size |
100 µg/vial |
Contents |
Antibody is lyophilized with 5 mg BSA, 0.9 mg NaCl, 0.2 mg Na2HPO4, 0.05 mg NaN3. *carrier free antibody available upon request. |
Concentration |
Reconstitute with 0.2 mL sterile dH2O (500 µg/ml final concentration). |
Storage |
At -20 °C for 12 months, as supplied. Store reconstituted antibody at 2-8 °C for one month. For long-term storage, aliquot and store at -20 °C. Avoid repeated freezing and thawing. |
Additional Information Regarding the Antigen
Gene |
TRPC5 |
Protein |
Short transient receptor potential channel 5 |
Uniprot ID |
Q9UL62 |
Function |
Thought to form a receptor-activated non-selective calcium permeant cation channel. Probably is operated by a phosphatidylinositol second messenger system activated by receptor tyrosine kinases or G-protein coupled receptors. Has also been shown to be calcium-selective (By similarity). May also be activated by intracellular calcium store depletion. |
Tissue Specificity |
Expressed in brain with higher levels in fetal brain. Found in cerebellum and occipital pole. |
Sub-cellular localization |
Cell membrane. |
Sequence Similarities |
Belongs to the transient receptor (TC 1.A.4) family. STrpC subfamily. TRPC5 sub-subfamily. |
Aliases |
Htrp 5 antibody|Htrp-5 antibody|Htrp5 antibody|Short transient receptor potential channel 5 antibody|Transient receptor potential cation channel subfamily C member 5 antibody|Transient receptor potential channel 5 antibody|Transient receptor protein 5 antibody|TRP 5 antibody|TRP-5 antibody|TRP5 antibody|TRPC 5 antibody|TrpC5 antibody|TRPC5_HUMAN antibody |
Application Details
| Application | Concentration* | Species | Validated Using** |
| Western blot | 0.1-0.5μg/ml | Human | AssaySolutio’s ECL kit |
AssaySolution recommends Rabbit Chemiluminescent WB Detection Kit (AKIT001B) for Western blot. *Blocking peptide can be purchased at $65. Contact us for more information
Anti- TRPC5 antibody, ASA-B1932, Western blotting
All lanes: Anti TRPC5 (ASA-B1932) at 0.5ug/ml
WB: Recombinant Human TRPC5 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
All lanes: Anti TRPC5 (ASA-B1932) at 0.5ug/ml
WB: Recombinant Human TRPC5 Protein 0.5ng
Predicted bind size: 50KD
Observed bind size: 50KD
Anti- TRPC5 antibody, ASA-B1932, Western blotting
All lanes: Anti TRPC5 (ASA-B1932) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 111KD
Observed bind size: 111KD
All lanes: Anti TRPC5 (ASA-B1932) at 0.5ug/ml
Lane 1: HELA Whole Cell Lysate at 40ug
Lane 2: U87 Whole Cell Lysate at 40ug
Lane 3: COLO320 Whole Cell Lysate at 40ug
Predicted bind size: 111KD
Observed bind size: 111KD
